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Image Search Results
Journal: Journal of Nanobiotechnology
Article Title: Peptide-modified phase-transition nanoparticles co-deliver FTO siRNA and Ce6 for sonodynamic metabolism-immunotherapy of melanoma
doi: 10.1186/s12951-025-03872-3
Figure Lengend Snippet: Gene silencing and glycolytic regulation of si-Ce6@tLyp-1-NPs. ( A ) Effect of UTMD in vitro ( B ) CEUS mode, n = 3 ( C ) Schematic illustration of the B16F10 cells treated with si-Ce6@tLyP-1-NPs combined with US ( D ) CLSM observation of the localization of FAM-siRNA in B16-F10 cells after transfection with free siRNA, si-Ce6@NPs(+), si-Ce6@tLyP-1-NPs (+). The scale was 10 μm, and Phalloidin was the cytoskeleton. DAPI is the nucleus. ( E ) The relative mRNA expression of FTO in different groups of B16-F10 ( F , G ) The protein expression of FTO in different groups of B16-F10. ( H ) Volcano plot of DEGs. ( I ) Heat map of genes involved in the glycolytic pathway. ( J) Representative pathways enriched in the identified genes as determined by GSEA (NES= −1.4016, p-value = 0.0424). ( K , L ) The mRNA expression levels of HK2 and PGAM1 in the control group and B16-F10 cells treated with different methods. ( M ) Representative western blot images of HK2 and PGAM1 expression in control and treated B16-F10 cell groups. ( N , O ) Quantitative analysis of HK2 and PGAM1 protein expression. ( P ) Lactate levels in cell culture medium were measured
Article Snippet: CRT antibody (IF:1:300, 27298-1), FTO antibody (WB:1:2000, IHC:1:300, 68111-1),
Techniques: In Vitro, Transfection, Expressing, Control, Western Blot, Cell Culture